High background in ELISpot assays is associated with elevated levels of immune activation in HIV-1-seronegative individuals in Nairobi

Autor:	James Kiarie, Robert Y. Choi, Stephen C. De Rosa, Barbara A. Richardson, Carey Farquhar, Amy Y. Liu, Rose Kerubo-Bosire, Barbara Lohman-Payne, Brandon L. Guthrie
Rok vydání:	2018
Předmět:	0301 basic medicine education.field_of_study medicine.diagnostic_test business.industry ELISPOT Immunology Population CD38 Peripheral blood mononuclear cell 3. Good health Flow cytometry 03 medical and health sciences 030104 developmental biology 0302 clinical medicine Immune system Interferon medicine Immunology and Allergy 030212 general & internal medicine education business CD8 medicine.drug
Zdroj:	Immunity, Inflammation and Disease. 6:392-401
ISSN:	2050-4527
DOI:	10.1002/iid3.231
Popis:	INTRODUCTION Spontaneous interferon-γ (IFNγ) released detected by enzyme-linked immunospot (ELISpot) assays may be a biological phenomenon. Markers of immune activation levels were assessed as correlates of high background among individuals in Kenya. METHODS Couples concordantly seronegative for HIV-1 were enrolled. IFN-γ ELISpot assays were conducted and negative control wells were categorized as having either high or low background (≥50 and
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::35c1678812faa9f761cf1b109bb590dc https://doi.org/10.1002/iid3.231 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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