| Autor: |
Zhang Xin, He Zhang, Xie Yixian, Peng Jun, Yanxiang Qi, Pu Jinji |
| Rok vydání: |
2014 |
| Předmět: |
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| Zdroj: |
Physiological and Molecular Plant Pathology. 86:81-88 |
| ISSN: |
0885-5765 |
| DOI: |
10.1016/j.pmpp.2014.04.002 |
| Popis: |
Fusarium mangiferae is a major causal agent of mango malformation disease (MMD) worldwide. Rapid and accurate detection of the causal pathogen is the cornerstone of integrated disease management. In this paper, a real-time fluorescence loop-mediated isothermal amplification assay (RealAmp) was developed for quantitative detection of F. mangiferae in China. The LAMP primer set was designed based on a RAPD marker sequence and positive products were amplified only from F. mangiferae isolates, but not from any other species tested, showing a high specificity of the primer sets. The detection limit was approximately 2.26 × 10−4 ng/μl plasmid DNA when mixed with extracted mango DNA. Quantification of the pathogen DNA of MMD in naturally collected samples was no significant difference compared to classic real-time PCR Additionally, RealAmp assay was visual with an improved closed-tube visual detection system making the assay more convenient in diagnostics. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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