RAPHANUS SATIVUS LINN. A NEW ANTINOCICEPTIVE FOR DIABETIC NEUROPATHY IN RATS DETERMINED BY RANDALL SELITTO APPROACH
| Autor: | Sthitapragnya Panda, Sambit Kumar Sahoo |
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| Rok vydání: | 2019 |
| Předmět: | |
| Zdroj: | Asian Journal of Pharmaceutical and Clinical Research. :529-534 |
| ISSN: | 2455-3891 0974-2441 |
| Popis: | Objective: The objective of the study was to evaluate the antinociceptive effect of Raphanus sativus Linn. using Randall Selitto method. Methods: Streptozotocin, lard, casein, cholesterol, DL-methionine, yeast powder, quercetin, thiobarbituric acid, 2-nitrobenzoic acid (5, 5, Dithiobis), hematoxylin, and hydrogen peroxide were used. A diet rich in fat content was fed to the animals for a period of 2 weeks. After a stabilization period of 2weeks, the treatment period started and continued for a period of 8weeks. The nociceptive parameters were assessed once a week by Randall Selitto method and hot plate test. After treatment, the animals were sacrificed, and antioxidant parameters were assessed using sciatic nerve homogenate and histopathological analysis of sciatic nerve. Results: Treatment R. sativus extract (RSE 100 mg/kg and 200 mg/kg) appreciably declined the levels of blood glucose in a dose-dependent manner, and it was comparable with standard quercetin. A significant increase in pain threshold levels was observed by the treatment RSE in hot plate method after the 4th week compared to diabetic control, and it was consistent until the end of treatment (p |
| Databáze: | OpenAIRE |
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