Effect of raw rice bran supplementation on rumen methanogen population density andin vitrorumen fermentation
| Autor: | Kenji Ito, Hiroaki Wakabayashi, Yuriko Ogawa, Ayaka Nishikawa, Masaaki Wakita, Tomomi Ban-Tokuda, Natsumi Fukushima, Hiroki Matsui, Shohei Yoneda, Ryota Yoshimi |
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| Rok vydání: | 2013 |
| Předmět: |
chemistry.chemical_classification
animal structures Bran biology digestive oral and skin physiology food and beverages Fatty acid Plant Science Butyrate biology.organism_classification Methanogen Rumen chemistry Biochemistry Propionate Fermentation Food science Agronomy and Crop Science Incubation Ecology Evolution Behavior and Systematics |
| Zdroj: | Grassland Science. 59:129-134 |
| ISSN: | 1744-6961 |
| Popis: | The effect of raw rice bran supplementation on in vitro rumen methane production, short-chain fatty acid (SCFA) production and profile, and the population density of methanogenic archaea was studied using batch cultures of rumen fluid. The experimental batch culture conditions were as follows: a control group containing a substrate without supplements; substrates supplemented with 5, 10 or 15% raw rice bran; and a culture treated with an antimethanogenic agent, bromoethanesulfonate (BES), as a positive control. The methanogen population density in the cultures was determined by methanogen-specific real-time polymerase chain reaction (PCR) assay. The BES treatment completely inhibited methane production. Supplementation with 10% raw rice bran inhibited methane production by 18% after a 24-h incubation (P > 0.05). No significant difference in total SCFA levels was observed between all the treatments, although the SCFA profiles were altered. Whereas the proportion of butyrate increased significantly, the proportion of propionate decreased significantly in the 10% rice bran treatment (P |
| Databáze: | OpenAIRE |
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