EVALUATING THE FEASIBILITY OF DETERMINING TOTAL PLASMA HOMOCYSTEINE AS HOMOCYSTEIC ACID USING ION-EXCHANGE CHROMATOGRAPHY

Autor:	Michael Lowry, Mark E. Arcaro, Jinmo Huang
Rok vydání:	2002
Předmět:	Performic acid Chromatography Homocysteine Clinical Biochemistry Ion chromatography Pharmaceutical Science Biochemistry Homocysteic acid Analytical Chemistry chemistry.chemical_compound chemistry Ninhydrin Blood plasma Derivatization Quantitative analysis (chemistry)
Zdroj:	Journal of Liquid Chromatography & Related Technologies. 25:759-770
ISSN:	1520-572X 1082-6076
DOI:	10.1081/jlc-120003033
Popis:	Total homocysteine content in plasma has been linked to the risk of occlusive vascular disease. Plasma homocysteine is present in multiple forms. Current methods for total plasma homocysteine analysis use reducing agents to convert other homocysteine-related compounds to homocysteine before analysis. Due to the incompleteness of reduction and the possibility of re-oxidation for homocysteine, accurate determination of total plasma homocysteine is not always achievable. In this research, performic acid is used to oxidize homocysteine-related compounds to homocysteic acid. The oxidized samples are separated and analyzed for homocysteic acid by ion-exchange chromatography with post-column ninhydrin derivatization and "spectrometric detection. The evaluation of performic acid oxidation on several homocysteine-related compounds and the feasibility of determining plasma homocysteine as homocysteic acid are studied.
Databáze:	OpenAIRE
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