Probiotic Lactobacillus Species Strengthen Intestinal Barrier Function and Tight Junction Integrity in Experimental Necrotizing Enterocolitis
| Autor: | Catherine J. Hunter, Carrie Yuan, Justyna S. Grothaus, Joseph D. Nicolas, Brian P. Blackwood, Douglas R. Wood |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
biology medicine.disease biology.organism_classification 3. Good health law.invention Microbiology 03 medical and health sciences Probiotic 030104 developmental biology Lactobacillus rhamnosus law In vivo Intensive care Lactobacillus Necrotizing enterocolitis medicine Barrier function Lactobacillus plantarum |
| Zdroj: | Journal of Probiotics & Health. |
| ISSN: | 2329-8901 |
| DOI: | 10.4172/2329-8901.1000159 |
| Popis: | Necrotizing enterocolitis (NEC) is a serious intestinal disease that occurs in newborn infants. It is associated with major morbidity and affects 5% of all infants admitted to neonatal intensive care units. Probiotics have variable efficacy in preventing necrotizing enterocolitis. Tight junctions (TJ) are protein complexes that maintain epithelial barrier integrity. We hypothesized that the probiotics Lactobacillus rhamnosus and Lactobacillus plantarum strengthen intestinal barrier function, promote TJ integrity, and protect against experimental NEC. Both an in vitro and an in vivo experimental model of NEC were studied. Cultured human intestinal Caco-2 cells were pretreated with L. rhamnosus and L. plantarum probiotics. TJ were then disrupted by EGTA calcium switch or LPS to mimic NEC in vitro. Trans-epithelial resistance (TER) and flux of fluorescein isothiocynate dextran was measured. TJ structure was evaluated by ZO-1 immunofluorescence. in vivo effects of ingested probiotics on intestinal injury and ZO-1 expression were assessed in a rat model of NEC infected with Cronobacter sakazaki (CS). Caco-2 cells treated with individual probiotics demonstrated higher TER and lower permeability compared to untreated cells (p |
| Databáze: | OpenAIRE |
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