Peanut Allergens Attached with p-Aminobenzamidine Are More Resistant to Digestion than Native Allergens
| Autor: | Shawndrika Reed, Dunhua Zhang, Si-Yin Chung |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
medicine.disease_cause
01 natural sciences chemistry.chemical_compound 0404 agricultural biotechnology Allergen Western blot immune system diseases otorhinolaryngologic diseases medicine biology medicine.diagnostic_test Chemistry 010401 analytical chemistry food and beverages 04 agricultural and veterinary sciences respiratory system Trypsin 040401 food science respiratory tract diseases 0104 chemical sciences Blot Biochemistry Glycine Digestive enzyme biology.protein Glutaraldehyde Digestion medicine.drug |
| Zdroj: | Food and Nutrition Sciences. :1352-1363 |
| ISSN: | 2157-9458 2157-944X |
| Popis: | Despite being known as resistant proteins, peanut allergens (Ara h 1 and Ara h 2) can be digested and cause allergic reactions. Making the allergens more resistant to digestion may aid in non-absorption and excretion of the allergens. Our objectives were to make Ara h 1 and Ara h 2 more resistant to digestion and test them in a model system using trypsin as the digestive enzyme. The resistant allergens were prepared by covalently attaching p-aminobenzamidine (pABA), a protease inhibitor, to peanut allergens in an extract or on a PVDF membrane using glutaraldehyde, and were then tested for resistance to trypsin digestion. SDS-PAGE and Western blot were performed to determine the allergenic capacity of the modified allergens. A control was prepared using glycine instead. Results showed that Ara h 2, when covalently attached with pABA, was more resistant to trypin digestion than the native allergen. Similarly, Ara h 1, prepared on a PVDF membrane and treated with pABA, displayed a resistance to trypsin digestion. Treatment of the allergens with glycine (a control) instead of pABA showed that the modified allergens were as digestible as native allergens. Blot assays showed that the pABA-treated allergens exhibited a lower allergenic capacity than native allergens. It was concluded that pABA, when attached to peanut allergen Ara h 1 or Ara h 2, inhibited digestion of the allergen by trypsin and reduced their allergenic capacity as well. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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