Enrichment of ω-3 Polyunsaturated Fatty Acids of Sardine Cannery Effluents Using Lipozyme™: Optimization of Reaction Conditions
| Autor: | Guilhem Marie-Christine, Schmitt-Rozieres Murielle, Phan Tan Luu Roger, Comeau Louis-Claude |
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| Rok vydání: | 2000 |
| Předmět: |
chemistry.chemical_classification
Chromatography Central composite design Sardine Fatty acid Alcohol Biochemistry Eicosapentaenoic acid Catalysis chemistry.chemical_compound chemistry Docosahexaenoic acid Yield (chemistry) lipids (amino acids peptides and proteins) Biotechnology Polyunsaturated fatty acid |
| Zdroj: | Biocatalysis and Biotransformation. 18:355-371 |
| ISSN: | 1029-2446 1024-2422 |
| Popis: | Enrichment of n-3 polyunsaturated fatty acids from sardine cannery effluents upon enzymatic esterification by Lipozyme® was optimized in batch and in continuous processes. In these processes, the yield of docosahexaenoic acid (DHA) (Y1) and the yield of eicosapentaenoic acid (EPA) (Y2), in the residual acid fraction were maximized. In batch, a two-factor Doehlert design was used to study the effects of temperature and ratio of fatty acid to alcohol. Second order polynomial regression models for Y1 and Y2 were postulated to generate response surfaces. After esterification the fraction of fatty acid was enriched to 70% with DHA or to 44% with EPA. In a continuous process, a three-factor central composite design was employed to study the effects of temperature, ratio of fatty acid to alcohol and flow rate. Second order polynomial regression models for Y1 and Y2 were used to generate response surfaces. After esterification, a quantity of DHA close to 30% and 17% of EPA. |
| Databáze: | OpenAIRE |
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