TISSUE PLASMINOGEN ACTIVATOR PRODUCTION BY MONOCYTES IN VENOUS THROMBOLYSIS
| Autor: | Andrew D. R. Northeast, L. G. Bobrow, K. S. Soo, K. G. Burnand, L. C. Happerfield |
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| Rok vydání: | 1996 |
| Předmět: |
Pathology
medicine.medical_specialty integumentary system Endothelium business.industry In situ hybridization medicine.disease Thrombosis Inferior vena cava Peripheral blood mononuclear cell Tissue plasminogen activator Pathology and Forensic Medicine Venous thrombosis medicine.anatomical_structure medicine.vein cardiovascular system medicine cardiovascular diseases Thrombus business circulatory and respiratory physiology medicine.drug |
| Zdroj: | The Journal of Pathology. 178:190-194 |
| ISSN: | 1096-9896 0022-3417 |
| DOI: | 10.1002/(sici)1096-9896(199602)178:2<190::aid-path454>3.0.co;2-3 |
| Popis: | Laminated occlusive thrombus was induced in the rat inferior vena cava (IVC) by a distal stenosis and injection of thrombin. Immunocytochemistry was performed on serial cryostat sections of the thrombus for tissue plasminogen activator (tPA) and a variety of phenotype markers for mononuclear cells. There was little tPA in 2-day-old thrombus. However, tPA was present in significant quantities in 1- and 2-week-old thrombus. Most of the staining for tPA was associated with monocytes, which had infiltrated the thrombus in large numbers. No caval endothelium was seen in these sections. By 4 weeks, the IVC had re-canalized and new endothelium had formed; tPA staining was weakly positive in the endothelium and smooth muscle. In situ hybridization with a digoxigenin-labelled RNA probe confirmed the monocytes as the main source of tPA. This study shows that large numbers of infiltrating monocytes are present in venous thrombosis and that they are the main source of tPA. |
| Databáze: | OpenAIRE |
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