Elektrochemilumineszenz-Bioassays können Fluoreszenzassays mithilfe eines wasserlöslichen Luminolderivats übertreffen
| Autor: | Antje J. Baeumner, Axel Jacobi von Wangelin, Simone Rink, Axel G. Griesbeck, Michael Mayer, Moritz Vollmer, Shigehiko Takegami, Silja Schulte, Axel Duerkop, Michael Neumeier |
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| Rok vydání: | 2017 |
| Předmět: |
Detection limit
Bioanalysis Chromatography 010405 organic chemistry chemical and pharmacologic phenomena General Medicine 010402 general chemistry Photochemistry 01 natural sciences Fluorescence 0104 chemical sciences Luminol law.invention chemistry.chemical_compound chemistry law Yield (chemistry) Solubility Biosensor Chemiluminescence |
| Zdroj: | Angewandte Chemie. 130:414-418 |
| ISSN: | 0044-8249 |
| DOI: | 10.1002/ange.201708630 |
| Popis: | The most efficient and commonly used electrochemilumi-nescence (ECL) emitters are luminol, [Ru(bpy)3]2+ and derivatives thereof. Luminol stands out due to its low excitation potential, yet applications are limited by the insolubility under physiological conditions. The water-soluble m-carboxy luminol was synthesized in 15% yield and exhibited high solubility under physiological conditions and afforded a four-fold ECL signal increase (vs. luminol). Entrapment in DNA-tagged liposomes enabled a DNA assay with a detection limit of 3.2 pmol L-1 which is 150 times lower than the corresponding fluorescence approach. This remarkable sensitivity gain and the low excitation potential establish m-carboxy luminol as a superior ECL probe with direct relevance to chemiluminescence and enzymatic bioanalytical approaches. |
| Databáze: | OpenAIRE |
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