| Autor: | Marie-Pierre Fontaine-Aupart, S. Tosti, Alain Brun, G. Roger, F. Tfibel, M. Enescu, B. Arrio, P. Georges, Elvire Guiot, G. Johannin, Fabienne Merola |
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| Rok vydání: | 2000 |
| Předmět: |
Fluorophore
Sociology and Political Science Clinical Biochemistry Analytical chemistry Fluorescence correlation spectroscopy Fluorescence in the life sciences Biochemistry Fluorescence Green fluorescent protein Clinical Psychology chemistry.chemical_compound chemistry Two-photon excitation microscopy Microscopy Fluorescein Law Spectroscopy Social Sciences (miscellaneous) |
| Zdroj: | Journal of Fluorescence. 10:413-419 |
| ISSN: | 1053-0509 |
| DOI: | 10.1023/a:1009490816254 |
| Popis: | We report on the application of fluorescence correlation microscopy under two-photon excitation of fluorophores of biological interest: FITC–dextran (MW, from 20 to 150 kDa), green fluorescent protein (MW, 27 kDa), and fluorescein (MW, 330 Da). Under these experimental conditions, the translational diffusion coefficients of these molecules in aqueous solutions derived from the fluorescence intensity autocorrelation function were determined for the first time and were found to be 24 × 10−7, 8.2 × 10−7, and 3 × 10−7 cm2 s−1 for 150-kDa FITC–dextran, green fluorescent protein, and fluorescein, respectively. These results are discussed in connection with previously reported results obtained by different methods. The great sensibility of the system has been applied to single-molecule detection of the smaller fluorophore, fluorescein. |
| Databáze: | OpenAIRE |
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