In utero complementation of a neural crest-derived melanocyte defect using cell directed gene transfer

Autor:	Yi Li, Arturo Incao, Dawn E. Watkins-Chow, Karen J. Dunn, William J. Pavan
Rok vydání:	2001
Předmět:	Genetics Genetically modified mouse animal structures Tyrosinase Neural crest Cell Biology Biology Melanocyte Embryonic stem cell Molecular biology Endocrinology medicine.anatomical_structure In utero embryonic structures Gene expression medicine Dopachrome tautomerase
Zdroj:	genesis. 30:70-76
ISSN:	1526-968X 1526-954X
DOI:	10.1002/gene.1035
Popis:	Summary: This study describes an in utero approach for overexpressing genes in a cell-type directed manner. It uses an avian leukosis retroviral expression system coupled with a transgenic mouse line expressing the viral receptor tv-a from a tissue-specific promoter (RCAS-TVA system) (Federspiel et al., 1994, and reviewed in Fisher et al., 1999). A transgenic mouse line was generated expressing tv-a from the Dopachrome tautomerase promoter (DCT-tv-a) in embryonic melanocyte precursors (melanoblasts). RCAS virus encoding β-galactosidase (RCAS-LacZ) or tyrosinase (RCAS-Tyr) was injected in utero into embryonic day 12.5 albino (tyrosinase inactive) mouse embryos. Animals were analyzed for β-galactosidase activity or tyrosinase activity (hair pigmentation). RCAS gene expression was detected in 44% and 25% of the transgenic mice, respectively. We demonstrate the RCAS-TVA system coupled with the DCT-tv-a line of mice can be used for in utero infection. genesis 30:70–76, 2001. © 2001 Wiley-Liss, Inc.
Databáze:	OpenAIRE
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