Physicochemical analysis of a protein with nerve growth activity from the muscle tissue of Rana ridibunda

Autor:	D. B. Mirkhodzhaev, I. I. Parilis, L. Ya. Yukel'son, Aripdzhanov Aa, R. S. Salikhov, D. Kh. Khamidov
Rok vydání:	1992
Předmět:	Muscle tissue chemistry.chemical_classification Molecular mass Phylogenetic tree Chemistry Lysine Plant Science General Chemistry General Biochemistry Genetics and Molecular Biology Nerve growth factor medicine.anatomical_structure Rana ridibunda Biochemistry Valine medicine Glycoprotein
Zdroj:	Chemistry of Natural Compounds. 28:217-221
ISSN:	1573-8388 0009-3130
DOI:	10.1007/bf00630181
Popis:	A protein nerve growth factor has been isolated in the pure form from the muscles of the lake frogRana ridibunda. The individuality of the substance obtained has been shown, its molecular mass (106.0–110.0 kDa) and pI value (9.2–9.4) have been determined, and lysine and valine have been found as the N- and C-terminal amino acid residues of its polypeptide chain. In view of the detection of the presence of sugars (about 1%), the substance has been identified as a glycoprotein. Its absorption spectra in the UV region has been obtained and the amino acid composition of the isolated protein has been determined. Information on the amino acid composition of various proteins with nerve growth activity has been subjected to comparative analysis with the aid of computer programs, and on this base their phylogenetic “tree” has been constructed.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::35342da81a1fe3932d481f4130b12fae https://doi.org/10.1007/bf00630181 Zobrazit plný text záznamu Full text from SpringerLink