| Autor: | Yumiko Kato, Yukiko Kawaguchi, Noboru Hiraoka, Jung I. Chang |
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| Rok vydání: | 2001 |
| Předmět: | |
| Zdroj: | Plant Cell, Tissue and Organ Culture. 67:243-249 |
| ISSN: | 0167-6857 |
| DOI: | 10.1023/a:1012739624062 |
| Popis: | Corydalis ambigua, a perennial herb of the family Papaveraceae, was micropropagated through somatic embryogenesis starting from sliced tubers. Somatic embryos were proliferated on Linsmaier and Skoog medium of a half strength containing 2% sucrose and 0.1 μM indole-3-acetic acid or indole-3-butyric acid solidified with 0.2% Gelrite. Somatic embryos were germinated and grew on plant growth regulator-free White's medium supplemented with 2% sucrose and 0.8% agar in the dark at 0–4°C for 6 months to give rise to microtubers that could be potted out of culture tubes. Three strains of micropropagated plants cultivated outdoors for 5 years gave different tetrahydroprotoberberine-type alkaloids pattern, respectively. The variation of tetrahydroprotoberberine-type alkaloid (corybulbine, corydaline and cavidine) content within a strain was not significantly different from that of the corresponding alkaloid in the wild plants. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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