Comparison of three human-murine heteromyeloma cell lines for formation of human hybridomas after electrofusion with human peripheral blood lymphocytes from meningococcal cases and carriers

Autor:	A. Delvig, Sigbert Jahn, J. F. Wang, Mark Achtman, Ralf W. Glaser, Koumaré B
Rok vydání:	1995
Předmět:	Human immunoglobulins biology Immunology General Medicine biology.organism_classification medicine.disease Virology Peripheral blood Electrofusion Cell culture medicine Homologous chromosome biology.protein Immunology and Allergy Antibody Meningitis Bacteria
Zdroj:	Human Antibodies. 6:42-46
ISSN:	1875-869X 1093-2607
DOI:	10.3233/hab-1995-6201
Popis:	Peripheral blood lymphocytes from meningitis patients and healthy meningococcal carriers were fused by electrofusion with the three human-murine heteromyeloma cell lines CB-F7, K6H6B5 and H7NS. 934 hybridomas producing human immunoglobulins were obtained in 30 fusions. Heteromyeloma K6H6B5 yielded a significantly higher proportion of hybridomas producing IgG antibodies than did the two other cell lines. CB-F7 and K6H6B5 yielded comparable numbers of hybridomas whose supernatants reacted with homologous bacteria, whereas the cell line H7NS was less efficient.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::352f693bffb8d3a2d684440313282fca https://doi.org/10.3233/hab-1995-6201 Zobrazit plný text záznamu