N,N-Dimethyl-D-ribo-phytosphingosine Modulates Cellular Functions of 1321N1 Astrocytes

Autor: Ji-Yeong Jo, Dong-Soon Im, Mijin Han, Santosh J. Sacket, Sung-Mee Lim, Kyeok Kim, Yun-Kyung Lee, Hyo-Lim Kim
Rok vydání: 2007
Předmět:
Zdroj: Biomolecules and Therapeutics. 15:73-77
ISSN: 1976-9148
DOI: 10.4062/biomolther.2007.15.2.073
Popis: N,N-Dimethyl-D-ribo-phytosphingosine (DMPH) is an N-methyl derivative of sphingosine. In the present paper, we studied effects of DMPH on intracellular Ca concentration, pH, glutamate uptake, and cell viability in human 1321N1 astrocytes. DMPH increased intracellular Ca concentration and cytosolic pH significantly in a dose-dependent manner. DMPH also inhibited glutamate uptake by 1321N1 astrocytes. Finally, treatment of cells with DMPH for 24 h reduced viability of cells largely and concentration-dependently. In summary, DMPH increased intracellular Ca concentration and pH, inhibited glutamate uptake and evoked cytotoxicity in 1321N1 astrocytes. Our observations with DMPH in the 1321N1 astrocytes would enhance understanding of DMPH actions in the brain.
Databáze: OpenAIRE