Platelet-Activating Factor Raises Airway and Vascular Pressures and Induces Edema in Lungs Perfused with Platelet-Free Solution1–3
| Autor: | Yuhei Hamasaki, Sami I. Said, Tsutomu Saga, Mohammad Mojarad, Hsin-Hsiung Tai |
|---|---|
| Rok vydání: | 1984 |
| Předmět: |
Pulmonary and Respiratory Medicine
medicine.medical_specialty Lung Platelet-activating factor Prostacyclin respiratory system Pulmonary edema medicine.disease Pulmonary hypertension Thromboxane B2 Thromboxane A2 chemistry.chemical_compound Endocrinology medicine.anatomical_structure Airway resistance chemistry Internal medicine Anesthesia medicine lipids (amino acids peptides and proteins) circulatory and respiratory physiology medicine.drug |
| Zdroj: | American Review of Respiratory Disease. 129:742-746 |
| ISSN: | 0003-0805 |
| DOI: | 10.1164/arrd.1984.129.5.742 |
| Popis: | The effects of synthetic platelet-activating factor (PAF) on guinea pig lung were examined in isolated lungs perfused with platelet-free Krebs-Ringer solution. When PAF (1 microgram) was injected into the pulmonary artery (PA), it markedly increased airway pressure (maximal increase, 84.7%) and moderately raised PA pressure (maximal increase, 22.8%). The same dose also provoked a massive (29-fold) release of thromboxane B2 (TXB2), the stable metabolite of TXA2, into the perfusate, beginning before the increases in airway and PA pressures. The concentration of 6 keto-PGF1 alpha, the stable metabolite of prostacyclin, also increased (to 5 times control levels) about 70 s after peak release of TXB2. Indomethacin completely blocked TXB2 release, reduced the magnitude of airway pressure increase by 79%, and shortened its duration, as well as the duration of the PA pressure rise. Larger concentrations of PAF (3 and 10 micrograms) produced even greater increments in airway and PA pressures, but these were only moderately attenuated by indomethacin. Also, PAF increased extravascular lung water, as evidenced by increases in wet/dry lung weight and lung/body weight ratios. In a concentration of 0.1 microgram, PAF had no effects on airway or PA pressures, nor did it stimulate TXB2 or 6-keto-PGF1 alpha release. Lyso-PAF was similarly ineffective. We conclude that PAF induces airway constriction, pulmonary hypertension, and pulmonary edema in guinea pig lung independently of platelets. These effects are associated with stimulated synthesis of TXA2, but the mechanisms of their production remain to be determined. |
| Databáze: | OpenAIRE |
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