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SUMMARY A homogeneous substance has been isolated from blood which inhibits bovine testicular hyaluronidase. It has been found to contain amino acids and hexosamine and gives the carbazole color reaction for uranic acid. It contains less than 0.1 per cent sulfur as sulfate, less than 0.2 per cent phosphorus, and less than 5 per cent lipides. No evidence for the presence of heparin was found. The molecular weight was estimated at 100,000, the mobility in Verona1 buffer at pH 8.6, p = 0.1, was -5.4 X 10-S sq. cm. per second per volt, the sedimentation constant was .so,~ = 3.75, and the diffusion constant was DZo = 3.4 f 0.3 x 10-’ sq. cm. per second. BIBLIOGRAPHY 1. Dorfman, A., Ann. New York Acad. SC., 62, 1098 (1950). 2. Glick, D., J. Mt. Sinai Hosp., 17. 207 (1950). 3. Mathews, M. B., and Dorfman, A., Physiol. Rev., in press. 4. Dorfman, A., Ott, M. L., and Whitney, R., J. Biol. Chem., 174, 621 (1948). 5. Glick, D., and Moore, D. H., Arch. Biochem., 19, 173 (1948). 1 The authors are indebted to Dr. Carol Spurling for aid in these studies. by guest, on July 20, 2011www.jbc.orgDownloaded from |
