Activators of spleen glucocerebrosidase from controls and patients with various forms of Gaucher's disease
| Autor: | Alakananda Basu, Robert H. Glew, Lydia B. Daniels, Linda S. Clark |
|---|---|
| Rok vydání: | 1984 |
| Předmět: |
chemistry.chemical_classification
medicine.medical_specialty Activator (genetics) Cell Biology Phosphatidylserine Phosphatidic acid medicine.disease Biochemistry chemistry.chemical_compound Enzyme activator Gaucher's disease Endocrinology Enzyme chemistry Phosphatidylcholine Internal medicine medicine Molecular Biology Glucocerebrosidase |
| Zdroj: | Journal of Biological Chemistry. 259:1714-1719 |
| ISSN: | 0021-9258 |
| DOI: | 10.1016/s0021-9258(17)43465-x |
| Popis: | Glucocerebrosidase from normal human spleen, and spleen from cases of neurologic (types 2 and 3) and nonneurologic (type 1) Gaucher's disease, was delipidated and inactivated by extraction from membranes with sodium cholate and ice-cold 1-butanol. Control glucocerebrosidase was stimulated markedly by large quantities (20-30 micrograms/assay) of phosphatidylserine (PS), or by a combination of smaller amounts (1-2 micrograms) of PS and 3 micrograms of a heat-stable factor (HSF) derived from the spleen of a patient with Gaucher's disease. The residual glucocerebrosidase from a nonneurologic case, but not a neurologic case, was also responsive to PS and HSF. The combination of HSF and PS decreased the Km of the normal enzyme for 4-methylumbelliferyl-beta-D-glucopyranoside from 8.0 to 1.6 mM. These effectors also increased the reactivity of glucocerebrosidase to the inhibitor conduritol B epoxide; HSF alone had no effect (t1/2 = 19 +/- 0.5 min) whereas the maximum rate of inactivation (t1/2 = 4.0 min) by conduritol B epoxide was achieved in the presence of a mixture of PS (1 microgram) and HSF (3 micrograms). Phosphatidylglycerol (PG) and phosphatidic acid, also acidic phospholipids, were effective activators of glucocerebrosidase. Varying the fatty acid composition of PG had little effect on its ability to stimulate glucocerebrosidase activity. However, in the case of phosphatidylcholine (PC), a weaker activator than PG or PS, fatty acid composition had a significant impact on the ability of this neutral lipid to activate glucocerebrosidase; dilinoleoyl-PC and dicaproyl-PC were moderately effective activators, but distearoyl-PC and dioleoyl-PC were almost totally inactive. The mono-, and di-, and trisialogangliosides (GM1, GD1, and GT1 were less than half as effective as PS as activators of glucocerebrosidase. These results indicate that acidic phospholipids and the heat-stable factor may both play a role in explaining the genetic heterogeneity of Gaucher's disease. |
| Databáze: | OpenAIRE |
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