| Autor: | Heini Frang, Jukka Hellman, Mika Scheinin, Tuomo Glumoff, Adrian Goldman |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
biology
medicine.drug_class Bioengineering General Medicine medicine.disease_cause biology.organism_classification Monoclonal antibody Applied Microbiology and Biotechnology Enterobacteriaceae Molecular biology Fusion protein Epitope law.invention Biochemistry law medicine Bacillus circulans Recombinant DNA Escherichia coli Biotechnology Cyclomaltodextrin glucanotransferase |
| Zdroj: | Biotechnology Letters. 22:1963-1966 |
| ISSN: | 0141-5492 |
| DOI: | 10.1023/a:1026766330471 |
| Popis: | Human α2(C2)-adrenergic receptor was expressed in Escherichia coli as a fusion protein with Bacillus circulans var. alcalophilus cyclomaltodextrin glucanotransferase. For the determination of the expression level (0.6 mg of solubilized fusion protein l−1 of E. coli culture), a two-site immunometric assay based on two monoclonal antibodies with different epitopes was developed. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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