Characterization of oligosaccharides from the non-reducing termini of keratan sulphate chains
| Autor: | J M Dickenson, Ian A. Nieduszynski, Thomas N. Huckerby |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
chemistry.chemical_classification
integumentary system Stereochemistry Cartilage General Chemistry Nuclear magnetic resonance spectroscopy Oligosaccharide equipment and supplies Keratan sulphate Extracellular matrix medicine.anatomical_structure Enzyme chemistry Biochemistry medicine Proton NMR General Materials Science Macromolecule |
| Zdroj: | Magnetic Resonance in Chemistry. 30:S134-S141 |
| ISSN: | 1097-458X 0749-1581 |
| DOI: | 10.1002/mrc.1260301322 |
| Popis: | Keratan sulphate (KS) chains prepared from both bovine tracheal rings and bovine femoral head cartilage were digested with the enzyme keratanase from Pseudomonas species and non-reducing terminal oligosaccharides were fractionated and purified using high-performance ion-exchange chromatography. Two oligosaccharides were fully characterized using one- and two-dimensional NMR spectroscopic methods. An oligosaccharide derived from bovine tracheal ring KS chains was shown to have the structure NeuAcα2–3Galβ1–4GlcNAc(6-SO4)β1–3Gal(6-SO4)β1–4GlcNAc(6-SO4)β1–3Gal-ol (1). An oligosaccharide derived from bovine articular cartilage after treatment with a keratanase preparation containing neuraminidase activity was shown to have the structure Galβ1–4GlcNAc(6-SO4)β1–3Gal(6-SO4)β1–4GlcNAc(6-SO4)β1–3Gal-ol (2). These non-reducing terminal structures are KS chain caps or terminators which, because of their accessibility, are likely to be involved in interactions with other macromolecules of the extracellular matrix. |
| Databáze: | OpenAIRE |
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