High-Performance Liquid Chromatography Analysis of Single-Origin Chocolates for Methylxanthine Composition and Provenance Determination

Autor:	Shannon E. Stitzel, Ryan E. Sours
Rok vydání:	2013
Předmět:	Chromatography Chemistry Analytical chemistry General Chemistry Linear discriminant analysis High-performance liquid chromatography Education Chemometrics Certified reference materials Calibration medicine Laboratory experiment Theobromine medicine.drug
Zdroj:	Journal of Chemical Education. 90:1227-1230
ISSN:	1938-1328 0021-9584
DOI:	10.1021/ed3003918
Popis:	A quantitative and qualitative high-performance liquid chromatography (HPLC) analysis of single-origin chocolates is presented as a laboratory experiment for an advanced analytical chemistry course. The quantitative portion involves the determination of caffeine and theobromine content by an internal standard calibration method and then a validation by comparison to a certified reference material. The qualitative component introduces students to chemometrics through the use of discriminant analysis to determine the provenance of an unknown single-origin chocolate sample.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::34d73909612ae9bb913c4c2817c64a1a https://doi.org/10.1021/ed3003918 Zobrazit plný text záznamu