Autor: Svetlana N. Khodyreva, T. M. Ivanova, G. V. Shishkin, I. O. Petruseva, I. V. Safronov, Olga I. Lavrik, M.I. Dobrikov, I. A. Drachkova
Rok vydání: 2001
Předmět:
Zdroj: Russian Journal of Bioorganic Chemistry. 27:330-339
ISSN: 1068-1620
Popis: ATP γ-amides containing in γ-N-position 1-methylpyrene, 9-methylanthracene, 10-chloro-9-methyl-anthracene, and 3-methylperylene residues were synthesized and characterized. These compounds were used as sensitizers of site-specific photomodification of the reconstituted elongating complex of the mammalian DNA polymerase β. The photomodification was carried out with the use of photoaffinity reagents, which were synthesized insituby the 5"-32P-labeled primers extension with photoreactive analogues of dCTP containing in the exo-N-position of cytosine various perfluoroarylazide groups. The effect of structures of the sensitizers and photoreactive reagents on the efficiency and selectivity of photocrosslinking of primers to the enzyme and template, as well as formation of a number of other photomodification products was studied. It was shown that the sensitizers containing 10-chloro-9-methylanthracene and 3-methylperylene residues allow one to obtain photocrosslinks under such irradiation conditions when photomodification in their absence is not essentially observed.
Databáze: OpenAIRE
Externí odkaz: