In vitro plant regeneration, flowering and fruiting from nodal explants of Andrographis lineata nees (Acanthaceae)
| Autor: | Rama Gopal Ghanta, Arifullah Mohammed, Kishore K. Chiruvella |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine food.ingredient Plant Science medicine.disease_cause 01 natural sciences 03 medical and health sciences food Murashige and Skoog medium Flower induction Pollen Botany medicine biology fungi Acanthaceae food and beverages Plant physiology biology.organism_classification Andrographis 030104 developmental biology Shoot Agronomy and Crop Science 010606 plant biology & botany Biotechnology Explant culture |
| Zdroj: | Journal of Crop Science and Biotechnology. 19:195-202 |
| ISSN: | 2005-8276 1975-9479 |
| Popis: | In the present study, in vitro propagation of tribal endemic medicinal plant, Andrographis lineata has been established using mature nodal explants. High frequency of regeneration (91.4%) was achieved on MS medium containing BA (3.0 mg L–1) along with IAA (0.2 mg L–1). Strikingly, irrespective of the season and collection period, we observed axillary flower induction and fruit formation from the above in vitro cultures supplemented with BA and NAA. Transition from the vegetative to the reproductive phase occurred within 2 months of culture, and importantly was influenced by factors such as sucrose and cytokinins. In vitro-regenerated flowers were morphologically identical to in vivo flowers. Furthermore, our scanning electron microscopic studies revealed that pollen external morphology of both in vitro and in vivo flowers were similar. The flowers self-fertilized and produced fruits in vitro. Elongated shoots rooted well on half-strength MS basal medium, and were successfully acclimatized to the garden conditions. Altogether, our established protocol can be utilized in plant breeding for the purpose of ex situ conservation, quick flowering, and fruit set. |
| Databáze: | OpenAIRE |
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