| Autor: |
null Humberto Estrella-Maldonado, null Jesús R. Mora Solís, null Cynthia G. Rodríguez-Quibrera |
| Rok vydání: |
2022 |
| Předmět: |
|
| Zdroj: |
International Journal of Science and Research Archive. 7:470-476 |
| ISSN: |
2582-8185 |
| DOI: |
10.30574/ijsra.2022.7.1.0223 |
| Popis: |
The research work covered the in vitro establishment of Persian lime nodal segments using a twig disinfection protocol, and their culture on Murashige and Skoog (MS) culture media supplemented with antibiotics (Estrepto-Ler® and Rifampicin) and fungicide (Chlorothalonil). The axillary buds production was performed cultured the nodal segments without contamination signs on MS culture media enriched with two cytokinins (6-BAP and Kinetin) at different concentrations. Results showed that 52 % of the nodal segments did not show signs of in vitro contamination when the twig submerged in 20% chlorine for 15 min during disinfection process and nodal segments were grown in MS culture medium supplemented with 2 mg L-1 of Rifampicin and fungicide Chlorothalonil. Interestingly, MS culture medium enriched with 6-BAP (0.5 mg L-1) + Kinetin (0.5 mg L-1) turned out to be the best combination to achieve that 78 % of nodal segments promoted 1 to 2 axillary buds. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
