| Popis: |
This study aimed to determine the protective effects of Thymoquinone (TQ), which is the most essential active compound of Nigella sativa, on the spermatological parameters of frozen‐thawed ram semen. Ejaculates were collected from five Sonmez rams. Ejaculates were extended with control (0 µg/mL TQ), 10, 25, 50 and 100 µg/mL TQ supplemented Tris-based extender. Then extended semen samples were equilibrated in a +4 °C cold cabinet for 2 h. After 2 h the samples loaded into 0.25 mL French straws. The straws were then frozen by liquid nitrogen vapour and stored in a liquid nitrogen container (-196 °C). For analyses, frozen samples were thawed in a water bath (37 oC for 30 s) and evaluated in terms of motility characteristics, plasma membrane acrosome integrity (PMAI), mitochondrial reactive oxygen spices level (MITOSOX+), lipid peroxidation level (BODIPY+), DNA damage and biochemical alterations (oxidative stress index, malondialdehide and glutathione). TQ 100 had higher total and progressive motility compared to control (P˂0.05). According to motility characteristics there were significant differences between the groups on curvilinear velocity (VCL), amplitude of lateral head displacement (ALH) and linearity (LIN; P˂0.05). The highest DNA damage was detected in control groups (P˂0.05). TQ50 had higher PMAI compared to control and TQ25 (P˂0.05). The lowest MITOSOX+ level was detected in TQ50 and TQ100 groups (P˂0.05). There were no significant differences on BODIPY+, oxidative stress index, malondialdehide and glutathione (P>0.05). According to results, it could be concluded that, supplementing 50 and 100µg/mL TQ to Tris extender that was used for ram semen cryopreservation, showed a positive effect on motility, PMAI and also decreased DNA damage and MITOSOX+. |
