| Autor: |
Jia Xudong, Gou Zhaozheng, Lu Yung-Cai, Zen Erzhen, Shi Fang, Wang Jain, Lia Xiaoqing, Zhou Yulin |
| Rok vydání: |
1987 |
| Předmět: |
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| Zdroj: |
XIth International Congress on Thrombosis and Haemostasis. |
| ISSN: |
2567-689X |
| DOI: |
10.1055/s-0038-1643029 |
| Popis: |
The mechanism of Rhaponticum Uniflorum DC in preventing coronary heart disease was studied in vivo and in vitro. TBA fluorescent method was used to determine lipid peroxides (Lpo) and double analysis method was used to determine glutathione peroxidase (GSH-Px) activity and fluorescent polarization of DPH probed membrane fluidity of smooth muscle cell (SMC).Rabbits were fed with high fat diet for 120 days. At the end of experiment, all the animals acquired hyperlipidemia and developed atheroma lesions in aorta and/or coronary. It was found that hyperlipidemia caused a rising of Lpo in blood (from 2.6±0.56 in control up to 8.48±3.28 nmol/ml) and in arterial wall (from 6.75±0.59 in control up to 31.94±4.20 nmol/g protein) and a decreasing of GSH-Px activity in arterial wall (from 0.210±0.095 down to 0.056±0.026 EU/g protein); concomitantly, an increase in microviscosity of arterial SMC membrane (from 1.93±0.04 in control up to 3.49±0.92 poise) which reflects a decrease in fluidity of SMC membrane. Lpo level was higher in plaque area (113.70±46.14 nmol/g protein) than in non-plaque area (58.32±12.69 nmol/g protein). GSH-Px activity level was lower in plaque area (0.0052±0.0014 EU/g protein) than in non-plaque area (0.015+0.0014 EU/g protein). Microviscosity of SMC membrane was higher in plaque area (2.92±0.35 poise) than in non-plaque area (2.26±0.24 poise, p |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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