Photometric Determination of Cu2+ Ion Using Octabromo-tetrakis(4-methylpyridyl)porphine

Autor:	Akimasa Iwado, Shuichi Enomoto, Masaki Mifune, Sinichiro Kamino
Rok vydání:	2012
Předmět:	inorganic chemicals Pharmacology chemistry.chemical_classification Calibration curve Chemistry Coefficient of variation Mole Analytical chemistry Pharmaceutical Science Molar absorptivity Sulfonic acid Ion Nuclear chemistry
Zdroj:	YAKUGAKU ZASSHI. 132:953-958
ISSN:	1347-5231 0031-6903
Popis:	Octabromo-tetrakis(4-methylpyridyl)porphine (OBTMPyP), an octabromonated compound with 4 pyrole rings of tetrakis(4-methylpyridyl)porphine, selectively forms a complex with Cu2+ ions at pH 2.0. When 3.6×10(-5) mol/L OBTMPyP was added to the reaction mixture, the calibration curve showed good linearity for Cu2+ ions ranging from 0.01-2.2 µg (addition of 1.0 mL). A good coefficient of variation (Cu2+ ions=1.5 µg (addition of 1.0 mL), n=10, 0.8%) was obtained. The molar absorption coefficient (e) based on Cu2+ ions was 8.5×10(4) L/mol•cm. This value was 6-fold greater than that determined with a clinical chemical analysis kit using the bathocuproine sulfonic acid method, which is a well-known method for spectrophotometric determination of the Cu2+ ion concentration. A deproteination method was successfully applied in the clinical analysis kit for determination of Cu2+ ion concentrations in control serum I, and the values determined using this method and the bathocuproine sulfonic acid method were almost the same.
Databáze:	OpenAIRE
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