| Autor: |
Liu Guisheng, Watcharapong Naraballobh, Tawatchai Teltathum, Molecular Breeding, Wuhan , China, Nanthana Pothakam, Korawan Sringarm, Pantaporn Supakankul, Bangkok Perdo-Che, Trisadee Khamlor, Supamit Mekchay, Worrarak Norseeda |
| Rok vydání: |
2020 |
| Předmět: |
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| Zdroj: |
Veterinary Integrative Sciences. 19:197-208 |
| ISSN: |
2629-9968 |
| Popis: |
Osteopontin (OPN) is a secreted phosphoprotein that is involved in the development of skeletal muscle and fat deposition. The objectives of this study were to identify the polymorphism of the OPN gene and to analyze the association of the OPN gene with intramuscular fat (IMF) content and fatty acid (FA) composition in pigs. Longissimus thoracis (LT) muscle samples taken from the 10-11th rib were collected from a total of 328 Duroc pigs. Genomic DNA samples were extracted from LT muscle tissues using the phenol-chloroform method. IMF content was measured using the ether extraction method and FA composition was measured by gas chromatography. The porcine OPN polymorphisms were identified by DNA sequencing and were genotyped using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. The association analysis of the OPN gene with IMF and FA composition traits was performed using a general linear model (GLM). Two polymorphic sites (OPN g.2442-2471indel and g.3836A>G) were found in the 5´-flanking region and intron 1 of the porcine OPN gene. The OPN g.2442-2471indel polymorphism was found to be significantly associated with IMF content and ω3 FA levels (PG polymorphism was significantly associated with the linolenic acid levels in the muscles of pigs (P |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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