Determination of mutacin activity and detection of mutA genes in Streptococcus mutans genotypes from caries-free and caries-active children

Autor:	Priscila Larcher Longo, R. O. Mattos-Graner, Marcia Pinto Alves Mayer
Rok vydání:	2003
Předmět:	Microbiology (medical) Genetics Genetic diversity biology Immunology biology.organism_classification Microbiology Streptococcus mutans Homology (biology) RAPD chemistry.chemical_compound chemistry Genotype General Dentistry Gene Genotyping DNA
Zdroj:	Oral Microbiology and Immunology. 18:144-149
ISSN:	0902-0055
Popis:	Relationships between genetic diversity, mutacin production and sensitivity to mutacins in Streptococcus mutans were evaluated in 19 clinical isolates from caries-free and caries-active children. Mutacin production was tested against 30 indicator strains; results showed significant variations in the inhibitory spectra of the clinical isolates. There was no association between the inhibitory spectrum of the infecting strain and the caries experience or the level of mutans streptococci infection of the host. Homology to the mutA gene coding for mutacin II was detected in one clinical isolate; none of the clinical isolates showed homology to the mutA genes coding for mutacins I or III. Genotyping by random amplified polymorphic DNA (RAPD) reactions grouped the isolates into three clusters, but no correlation was found between any of the clusters and mutacin activity, caries experience or level of mutans streptococci in the host.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::349f18a802437f95c08fec593977a20d https://doi.org/10.1034/j.1399-302x.2003.00042.x Zobrazit plný text záznamu Plný text