ChemInform Abstract: Simple Synthesis of Sialyllactose-Carrying Polystyrene and Its Binding with Influenza Virus

Autor: Suzuki Yasuo, Takashi Suzuki, Kazukiyo Kobayashi, Noritaka Matsubara, Tsukasa Muramatsu, Akiko Tsuchida
Rok vydání: 2010
Předmět:
Zdroj: ChemInform. 30
ISSN: 1522-2667
0931-7597
DOI: 10.1002/chin.199937302
Popis: Glycoconjugate polystyrenes bearing sialyllactose moieties were prepared via a simple method from a mixture of α2-6 and α2-3 linked sialyllactose isomers of bovine milk origin. The reducing end of sialyllactose was converted to an amino function with ammonium hydrogen carbonate and then coupled with p -vinylbenzoyl chloride. The resulting styrene derivative substituted with sialyllactose via an amide linkage was polymerized with ammonium peroxodisulfate and N,N,N,N -tetramethylethylenediamine in water at 30 °C. The interaction of the glycopolymer with influenza A and B viruses was investigated by three different methods. The glycopolymer inhibited the hemagglutination of influenza A virus (PR/8/34) and its activity was 103 times higher than that of the oligosaccharide itself. The cytopathic effect of virus-infected MDCK (Madine-Darby canine kidney) cells was inhibited by the glycopolymer. The homopolymer showed 102 times higher inhibitory activity than naturally-occurring fetuin. It was also found that various viruses could be trapped by the glycopolymer adsorbed on a polystyrene surface. The inhibitory and trapping activities of the glycopolymers were correlated with the sialyl linkage specificities of the virus strains.
Databáze: OpenAIRE
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