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Background: The evaluation of potential drugs is urgently needed against SARS-CoV2 and Lopinavir/ritonavir and Clarythromycin was considered as the drugs. Methods: Cytotoxicity test using MTT assay was done using human mesenchymal stem cells and drugs concentration used: 0.2; 2; 10; 100; 400 ppm for each Lopinavir, Clarithromycin and combining Lopinavir+Clarithromycin. We prepared Vero cells in 12-well plate for viral inoculation then given drugs with concentration: 1 ppm, 15 ppm, and 37.5 ppm of Lopinavir/ritonavir (200/50 mg), either 0.5 ppm, 4 ppm and 8 ppm of Chlarithromycin (500 mg) and 0.5 ppm, 5 ppm and 15 ppm of combination Lopinavir+Chlarithromycin. The qRT-PCR was performed to observe viral load. The observation were done in 24, 48, 72 hours after inoculation. Results: There is no cytotoxic effect in human mesenchymal stem cells. Testing with multilevel concentration 50% (CC50) showed cytotoxicity level (ppm) for each Lopinavir, Clarithromycin and Lopinavir+Clarithromycin are 3451.8 ppm, 2285.97 ppm and 12230.6 ppm. The morphology changes in Vero cells showed formation of giant cells, colony of detachment cells and cyto pathic effect. Antiviral activity or inhibition concentration of 50% to virus (IC50) value of each, Lopinavir, Clarithromycin and combination Lopinavir+Clarithromycin are 0.9757; 0.9246; and |
