Cellulose-binding proteins ofFibrobacter succinogenesand the possible role of a 180-kDa cellulose-binding glycoprotein in adhesion to cellulose
| Autor: | Emmanuel E. Egbosimba, Cecil W. Forsberg, Jianhua Gong |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
chemistry.chemical_classification
Fibrobacter succinogenes Binding protein Immunology General Medicine Biology Cellulose binding Polysaccharide biology.organism_classification Applied Microbiology and Biotechnology Microbiology Molecular biology chemistry.chemical_compound chemistry Biochemistry Genetics Cellulose Bacterial outer membrane Glycoprotein Molecular Biology Bacteria |
| Zdroj: | Canadian Journal of Microbiology. 42:453-460 |
| ISSN: | 1480-3275 0008-4166 |
| DOI: | 10.1139/m96-062 |
| Popis: | Fibrobacter succinogenes possesses seven cellulose-binding proteins (CBPs) of 40, 45, 50, 120, 180, 220, and 240 kDa. The 120-, 180-, 220-, and 240-kDa proteins were present in the outer membrane (OM), while the 40-, 45-, 50-, and 120-kDa proteins were either periplasmic or peripheral membrane proteins. The 120-kDa CBP, which was identified as endoglucanase 2, was a major component in both the OM and periplasm. Zymogram analysis for glucanases showed that the major membrane-associated CBPs, with the exception of endoglucanase 2, lacked endoglucanase activity. Affinity-purified antibodies against the 180-kDa CBP cross-reacted strongly with numerous cell envelope proteins of higher and lower molecular mass, including the previously characterized chloride-stimulated cellobiosidase. Treatment of the 180-kDa CBP and cell envelope proteins with periodate resulted in almost complete loss of antibody binding, suggesting that they possessed a common epitope that was carbohydrate in nature. Immunogold labelling of whole cells using antibodies against the 180-kDa CBP demonstrated that either the 180-kDa CBP or related proteins with a cross-reactive epitope were located at the cell surface. These epitopes were distributed uniformly on cells not bound to cellulose but congregated on the cell surface at sites of adhesion of cells to cellulose. Antibodies to the 180-kDa protein caused 62% inhibition of binding of F. succinogenes to crystalline cellulose, which provides evidence that either the 180-kDa CBP and (or) other related cross-reactive surface proteins have a role in adhesion to cellulose.Key words: cellulose, adhesin, adhesion, binding, Fibrobacter, succinogenes, rumen. |
| Databáze: | OpenAIRE |
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