Determining Ligand Path Through a Major Drug Transporter, AcrB, in Escherichia coli

Autor:	Fasahath Husain, Hiroshi Nikaido
Rok vydání:	2017
Předmět:	0301 basic medicine 03 medical and health sciences Membrane protein Chemistry 030106 microbiology medicine Assay standardization Computational biology Drug transporter medicine.disease_cause Ligand (biochemistry) Escherichia coli
Zdroj:	Methods in Molecular Biology ISBN: 9781493974528
DOI:	10.1007/978-1-4939-7454-2_10
Popis:	An experimental approach to detect the path a substrate takes through a complex membrane protein is described with emphasis on technical approach and theoretical considerations. The protocols for bacterial culture preparation, membrane protein purification, fluorescent assay standardization, data collection, and data analysis are provided. Useful software tools are recommended.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::346849a26f3b463f1bc86cd68687bf9b https://doi.org/10.1007/978-1-4939-7454-2_10 Zobrazit plný text záznamu