Immobilization of Cellulase from Bacillus subtilis UniMAP-KB01 on Multi-walled Carbon Nanotubes for Biofuel Production
| Autor: | Balakrishnan Kunasundari, Yong Hoo Peng, Sandrasekaran Naresh, Siew Hoong Shuit, Hwa Ng Qi, Yi Peng Teoh |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
biology
Filter paper Chemistry Gram-positive bacteria Thermophile 02 engineering and technology Bacillus subtilis Cellulase 010402 general chemistry 021001 nanoscience & nanotechnology biology.organism_classification 01 natural sciences Enzyme assay 0104 chemical sciences Hydrolysis biology.protein Food science 0210 nano-technology Gram |
| Zdroj: | IOP Conference Series: Materials Science and Engineering. 318:012008 |
| ISSN: | 1757-899X 1757-8981 |
| DOI: | 10.1088/1757-899x/318/1/012008 |
| Popis: | Bacillus subtilis UniMAP-KB01, a cellulase producer was isolated from Malaysian mangrove soil. Through morphological identification it was observed that the B. subtilis appears to be in rod shaped and identified as a gram positive bacterium. Growth profile of isolated B. subtilis was established by measuring optical density (OD) at 600 nm for every 1 hour intervals. Polymath software was employed to plot the growth profile and the non-linear plot established gave the precision value of linear regression, R2 of 0.9602, root mean square deviation (RMSD) of 0.0176 and variance of 0.0025. The hydrolysis capacity testing revealed the cellulolytic index of 2.83 ± 0.46 after stained with Gram's Iodine. The harvested crude enzyme after 24 hours incubation in carboxymethylcellulose (CMC) broth at 45°C and 100 RPM, was tested for enzyme activity. Through Filter Paper Assay (FPA), the cellulase activity was calculated to be 0.05 U/mL. The hydrolysis capacity testing and FPA shown an acceptable value for thermophilic bacterial enzyme activity. Thus, this isolated strain reasoned to be potential for producing thermostable cellulase which will be immobilized onto multi-walled carbon nanotubes and the cellulolytic activity will be characterized for biofuel production. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|