| Autor: |
Choi iok Wong, Elizabeth M. Wilson, Zhong Xun Zhou, Madhabananda Sar |
| Rok vydání: |
1994 |
| Předmět: |
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| Popis: |
Publisher Summary This chapter provides an overview of the androgen receptor (AR). AR is a ligand-activated transcriptional regulatory protein that mediates androgen-induced male sexual development and function. The chapter presents a schematic diagram that highlights the major functional domains of human AR. The centrally located DNA-binding domain contains nine highly conserved Cys residues common to the family of steroid receptors. This arrangement of Cys residues led to the confirmation of the requirement for zinc in the tertiary structure of this region of steroid receptors. It was recently noted that the AR protein is significantly stabilized by androgen binding. Pulse-chase experiments using 35S-labeled AR in transiently transfected monkey kidney COS cells showed rapid degradation of receptor in the absence of androgen (t1/2 = 1 hour at 37°C) or in the presence of nonandrogenic hormones. Androgen addition increased the AR protein half-life to 6 hours at 37°C. The sixfold androgen-induced stabilization of AR protein contrasts androgen-induced down-regulation of AR mRNA in tissues of the male reproductive tract. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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