Modulation of plasma antioxidant levels, glutathione S -transferase activity and DNA damage in smokers following a single portion of broccoli: a pilot study
| Autor: | Patrizia Riso, Marisa Porrini, Daniela Martini, Stefano Vendrame, Antonella Brusamolino, Cristian Del Bo, Gaia Bonacina |
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| Rok vydání: | 2013 |
| Předmět: |
chemistry.chemical_classification
Nutrition and Dietetics Antioxidant Vitamin C DNA damage medicine.medical_treatment food and beverages Absorption (skin) Plasma levels Peripheral blood mononuclear cell Biochemistry chemistry medicine Food science Agronomy and Crop Science Glutathione S-transferase activity Carotenoid Food Science Biotechnology |
| Zdroj: | Journal of the Science of Food and Agriculture. 94:522-528 |
| ISSN: | 0022-5142 |
| Popis: | BACKGROUND: Broccoli is a rich source of bioactive compounds (i.e. glucosinolates, carotenoids, vitamin C and folate) that may exert an antioxidant effect and reduce oxidative damage. The objective of this pilot study was to investigate the effect of broccoli consumption on carotenoids, vitamin C and folate absorption, glutathioneS-transferase (GST) activity, and oxidatively induced DNA damage in male smokers. METHODS: Ten healthy subjects consumed a single portion of steamed broccoli (250 g) with cooked pasta. Blood was drawn at baseline and at 3, 6 and 24 h from consumption. RESULTS: Broccoli significantly (P ≤ 0.01) increased plasma level of vitamin C and folate (+35% and 70%, respectively) at 3 h, and β-carotene (+8%) at 6 h. A modulation of GST activity occurred in plasma 6 h after broccoli consumption. A significant (P ≤ 0.01) reduction of the levels of H2O2-induced DNA damage (−18%) was observed in blood mononuclear cells 24 h after broccoli intake in GSTM1 positive, but not in GSTM1 null subjects. CONCLUSION: One portion of broccoli increased plasma antioxidant levels, modulated plasma GST activity and improved cell resistance against H2O2-induced DNA damage in healthy smokers. These results support the importance of consuming fruit and vegetable regularly. c � 2013 Society of Chemical Industry |
| Databáze: | OpenAIRE |
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