Structural analysis of water-soluble and -insoluble ?-glucans of whole-grain oats and barley
| Autor: | Päivi Tuomainen, Liisa Virkki, Maija Ylinen, Päivi Ekholm, Liisa Johansson |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
chemistry.chemical_classification
food.ingredient Molar mass Chromatography Polymers and Plastics Molecular mass Chemistry Organic Chemistry food and beverages 04 agricultural and veterinary sciences 02 engineering and technology 021001 nanoscience & nanotechnology 040401 food science Whole grains NMR spectra database 0404 agricultural biotechnology Avena food Water soluble Materials Chemistry Proton NMR 0210 nano-technology Glucan |
| Zdroj: | Carbohydrate Polymers. 58:267-274 |
| ISSN: | 0144-8617 |
| DOI: | 10.1016/j.carbpol.2004.06.041 |
| Popis: | Water-soluble and water-insoluble (1→3),(1→4)-β- d -glucans were isolated from whole-grain oats and barley and digested with lichenase. The oligosaccharides thus produced were analysed using anion-exchange chromatography with pulse-amperometric detection (HPAEC-PAD). The FT-IR, 1H NMR and solid-state 13C CP-MAS NMR spectra of the β-glucans were also measured. Analyses of the ratio of oligosaccharides with degrees of polymerisation 3 and 4 (DP3:DP4) showed small structural differences between oats and barley and between the water-soluble and water-insoluble β-glucans. The molar masses analysed using the SEC-HPLC method were 500,000 g/mol for the soluble β-glucans of both oats and barley and |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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