Expression of modified oxidase of D-aminoacids of Trigonopsis variabilis in methylotrophic yeasts Pichia pastoris
| Autor: | Michael A. Eldarov, E K Novikova, V. A. Redo |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Fungal protein
biology D-amino acid oxidase biology.organism_classification Applied Microbiology and Biotechnology Biochemistry Pichia pastoris law.invention Microbiology chemistry.chemical_compound Chitin chemistry law Chitinase Bacillus circulans Recombinant DNA biology.protein Expression cassette |
| Zdroj: | Applied Biochemistry and Microbiology. 47:32-37 |
| ISSN: | 1608-3024 0003-6838 |
| Popis: | Effective recombinant strains Pichia pastoris that produce functionally active hybrid of Trigonopsis variabilis D-aminoacids bond with chitin-connecting domain of chitinase A1 of Bacillus circulans (DAOcbd) were obtained. The dependence of DAOcbd production levels from production of the number of copies of “expression cassette” integrated in the AOX1 locus of recombinant strains was studied. It was indicated that synthesized DAOcbd may be easily purified and immobilized on chitin sorbents and possessed high specific activity. Produced strains and methods of their cultivation and DAOcbd extraction may be used for development of technologies of obtaining of biocatalyzers in technological processes of obtaining of 7-aminocepha-losporane acid. |
| Databáze: | OpenAIRE |
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