| Autor: |
Karam Chand, Shashi Bhusan Sudhakar, Raja Wasim Yousuf, Dhanavelu Muthuchelvan, Awadh Bihari Pandey, Kaushal Kishore Rajak, Bimalendu Mondal, Muthannan Andavar Ramakrishnan, Gopal R. Gowane, Arnab Sen, Sanchay Kumar Biswas |
| Rok vydání: |
2015 |
| Předmět: |
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| Zdroj: |
Small Ruminant Research. 124:137-139 |
| ISSN: |
0921-4488 |
| DOI: |
10.1016/j.smallrumres.2015.01.007 |
| Popis: |
Peste-des-petits-ruminants and bluetongue are two of the most important viral diseases of small ruminants. In the present study, a combined indirect enzyme-linked immunosorbent assay (CI-ELISA) was developed, which can be run parallelly on the same plate for simultaneous detection of antibodies against peste-des-petits-ruminants virus and bluetongue virus. A total of 503 goat sera samples were used for developing the CI-ELISA. The relative sensitivity and specificity of CI-ELISA for detection of antibodies against PPRV were 90% and 88.8%, respectively at cut off level of 34.52% positivity. In case of BTV, 95.9% relative sensitivity and 98.6% specificity values were obtained at cut-off level of 40.42% positivity. These results indicate that the single plate CI-ELISA reported here could be used as an alternate method to existing dual plate competitive ELISAs for diagnosis/sero-surveillance of both the diseases. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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