Mutagenicity of diesel engine exhaust in the Ames /Salmonella assayusing a direct exposure method

Autor:	Jean-Paul Morin, Frédéric Dionnet, Hasnaà Haddouk, Roy Forster, Stéphane Loriot, Mamadou Fall, Amadou Diouf
Rok vydání:	2011
Předmět:	Salmonella Diesel exhaust Chemistry Health Toxicology and Mutagenesis respiratory system medicine.disease_cause Diesel engine complex mixtures Pollution Ames test Gas phase Nitroreductase Direct exposure Environmental chemistry medicine Environmental Chemistry human activities Genotoxicity
Zdroj:	Toxicological & Environmental Chemistry. 93:1971-1981
ISSN:	1029-0486 0277-2248
Popis:	The aim of this study was to investigate the potential mutagenic activity of diesel engine exhaust in the Ames/Salmonella assay using a direct aerosol exposure system. So, TA 98 and TA 100 strains, with or without added S9 mix, were exposed to diesel emissions after varying degrees of filtration. Variants of these two strains, deficient in nitroreductase (TA 98NR and TA 100NR) or over-expressing O-Acetyl Transferase (YG 1024 and YG 1029), were also exposed to total (unfiltered) diesel exhaust to highlight the putative mutagenicity of any nitro-PAHs present in these emissions. Mutagenic activity of the diesel exhaust was demonstrated on Salmonella typhimurium, strains TA 100 and variants TA 100 NR and YG1029. The use of a particle filter did not modify the genotoxicity of the diesel emissions, indicating a major contribution of the gas phase to the mutagenicity of these diesel emissions. The prominent role of the particulate-associated nitro-polycyclic aromatic hydrocarbons (nitro-PAHs) claimed by some aut...
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::342ec2cfd5f7f4ce41f7293989c54c02 https://doi.org/10.1080/02772248.2011.619538 Zobrazit plný text záznamu