Influence of Bacterial Cellulose Gel Film Modification on Its Mechanical Properties and Ability to Covalently Bind Enzymes

Autor:	I. P. Grunyushkin, A. A. Devyataeva, D. A. Kadimaliev, A. N. Malafeev, Victor V. Revin
Rok vydání:	2021
Předmět:	chemistry.chemical_classification Polymers and Plastics Sodium periodate Infrared spectroscopy Aldehyde chemistry.chemical_compound Enzyme chemistry Bacterial cellulose Covalent bond Polymer chemistry Materials Chemistry Ceramics and Composites Titration Fourier transform infrared spectroscopy
Zdroj:	Polymer Science, Series B. 63:232-238
ISSN:	1555-6123 1560-0904
DOI:	10.1134/s1560090421030088
Popis:	The rules for modifying the gel film of bacterial cellulose without disturbing its film structure are optimized. Using FTIR spectroscopy and titration, it is shown that oxidation with sodium periodate makes it possible to obtain dialdehyde derivatives of gel films with different amounts of aldehyde groups. The conditions for immobilizing pepsin and β-galactosidase enzymes on modified gel films are selected. An analysis of IR spectra shows that the enzymes are retained on the gel film owing to formation of azomethine bonds between the amino groups of the protein and the aldehyde groups of the film. The efficiency of immobilization depends on the type of enzyme and the conditions of the grafting reaction; under the same conditions, the gel film binds more β-galactosidase than pepsin.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::341d7ac5c59b07be71a9ccd30b2e602d https://doi.org/10.1134/s1560090421030088 Zobrazit plný text záznamu Full text from SpringerLink