Multiple Components Rapidly Screened from Perilla Leaves Attenuate Asthma Airway Inflammation by Synergistic Targeting on Syk
| Autor: | Qi Hou, Wei Sun, Ziqian Zhang, Xuyu Li, Mingbao Lin, Chun-Suo Yao, Pei Ma, Jiqiao Yuan, Hui Yang, Fan Yannan, Shuyi Li |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Perilla frutescens biology Rosmarinic acid Immunology Syk hemic and immune systems Inflammation Pharmacology Perilla biology.organism_classification In vitro 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology 0302 clinical medicine chemistry In vivo 030220 oncology & carcinogenesis medicine Immunology and Allergy medicine.symptom Protein kinase C |
| Zdroj: | Journal of Inflammation Research. 13:897-911 |
| ISSN: | 1178-7031 |
| DOI: | 10.2147/jir.s281393 |
| Popis: | Background Perilla frutescens (L.) Britt., a classic medicinal plant, has been demonstrated to have anti-inflammatory and anti-allergic effects in asthma. Perilla leaves extract (PLE) exerted significant therapeutic effect against allergic asthma inflammation through Syk inhibition. But the active chemical ingredients from PLE are complex and unclear, it is difficult to fully elucidate its pharmacological mechanisms. Methods A method was established for rapid screening and characterization of active ingredients from PLE that targeted Syk, with which three potential active ingredients were identified. By using OVA-induced allergic asthma mouse model in vivo, OVA-induced human PBMCs inflammation model and DNP-IgE/BSA-induced RBL-2H3 cells model in vitro, the effects and mechanisms of PLE and its active components were evaluated. Results Using Syk-affinity screening method, roseoside (RosS), vicenin-2 (Vic-2) and rosmarinic acid (RosA) were identified from PLE. In vitro, PLE and its ingredients showed significant inhibitory activities against Syk, with their mixture (Mix, prepared by RosS, Vic-2 and RosA in accordance with their ratio in Syk-conjugated beads bound fraction) showing a stronger inhibitory activity. RosS, Vic-2 and RosA also showed significant effects on allergic asthma, and a synergistic effect of Mix was observed. Moreover, treatment with PLE, RosS, Vic-2, RosA, and Mix significantly inhibited the expression and phosphorylation of Syk, PKC, NF-κB p65, and cPLA2 in allergic mice lung tissue and in RBL-2H3 cells. Conclusion PLE may alleviate allergic airway inflammation partly through the multiple components synergistic targeting on Syk and its downstream inflammatory pathway. |
| Databáze: | OpenAIRE |
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