Improved gel-protected bilayers
| Autor: | David J. Walton, R. F. Costello, John Heptinstall, I. R. Peterson |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Chromatography
Chemistry Bilayer technology industry and agriculture Biomedical Engineering Biophysics Synthetic membrane General Medicine Valinomycin chemistry.chemical_compound Membrane Electrochemistry Gramicidin Agarose lipids (amino acids peptides and proteins) Lipid bilayer Biosensor Biotechnology |
| Zdroj: | Biosensors and Bioelectronics. 14:265-271 |
| ISSN: | 0956-5663 |
| DOI: | 10.1016/s0956-5663(99)00003-2 |
| Popis: | We report improved techniques for the fabrication of biomimetic membranes protectively sandwiched between two slabs of agarose gel and the evaluation of their characteristics relevant for use in a biosensor based on gated channel proteins. The gel protects the membrane from mechanical contact, from low-energy solvents and from drying out. The new methods allow the preparation in high yield of sensor cells with a front gel thickness less than 100 μm. During fabrication, gel-protected membranes of DOPC (dioleoyl phosphatidylcholine) readily incorporate valinomycin in functional form. DOPC membranes respond as expected and within a few minutes to aqueous solutions of gramicidin applied to the front gel layer. In long-term studies of protected membranes of the synthetic lipid PSPC, no failures were observed over runs of up to 3 weeks and the membrane leakage decreased, reaching within 7 days values comparable to those reported for unsupported bilayer lipid membranes. Initial attempts to incorporate functional nicotinic acetylcholine receptors have shown promise. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect