Rescue of streptomycin activity by piperine inMycobacterium tuberculosis

Autor:	Regiane Bertin de Lima Scodro, Eloísa G Sampiron, Rosilene Fressatti Cardoso, Letícia Sayuri Murase, João Vp Souza, Giovana F Costacurta, Aryadne Larissa de Almeida, Katiany Rizzieri Caleffi-Ferracioli, Laíse Adriane Hegeto, Vera Ld Siqueira, Leonora Lacerda Calsavara, Nathally Claudiane de Souza Santos
Rok vydání:	2021
Předmět:	Microbiology (medical) biology Absolute quantification biology.organism_classification Microbiology Molecular biology Mycobacterium tuberculosis Minimum inhibitory concentration chemistry.chemical_compound chemistry Streptomycin Mic values Piperine medicine Efflux Ethidium bromide medicine.drug
Zdroj:	Future Microbiology. 16:623-633
ISSN:	1746-0921 1746-0913
Popis:	Aim: To evaluate the modulatory effect of piperine (PIP) on streptomycin (SM) activity in Mycobacterium tuberculosis ( Mtb). Materials & methods: SM and PIP minimum inhibitory concentration (MIC) and combinatory activity were determined in Mtb H37Rv and in susceptible and resistant clinical isolates. Ethidium bromide accumulation assay and relative quantification of efflux pumps genes ( rv1258c, rv1218c and rv2942), after SM and SM+PIP combination exposure, were also performed. Results: PIP concentration of 25 μg/ml (1/4× MIC) was able to inhibit efflux pumps activity, to modulate SM activity in Mtb, and conducted changes in the relative quantification of efflux pumps genes. Conclusion: SM+PIP combination was able to rescue the SM-susceptible MIC values in SM-resistant Mtb.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::341402b944fa566a5d13b489aa2754bc https://doi.org/10.2217/fmb-2020-0124 Zobrazit plný text záznamu