Determination of Melamine Based on the Formation of an Insoluble Copper-Melamine Complex and Flame Atomic Absorption Spectrometry
| Autor: | Apichat Imyim, Nakara Bhawawet, Saowarux Fuangswasdi |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Detection limit
Calibration curve Biochemistry (medical) Clinical Biochemistry Analytical chemistry chemistry.chemical_element Biochemistry High-performance liquid chromatography Chloride Copper Analytical Chemistry Absorbance chemistry.chemical_compound chemistry Standard addition Electrochemistry medicine Melamine Spectroscopy medicine.drug |
| Zdroj: | Analytical Letters. 47:1931-1937 |
| ISSN: | 1532-236X 0003-2719 |
| DOI: | 10.1080/00032719.2014.888728 |
| Popis: | A copper-melamine complex was optimally synthesized by heating excess copper(II), as 50 m mol L−1 copper(II) chloride in 50% (v/v) methanol, and melamine at 80°C. The amount of residual copper(II) in solution after removal of the copper-melamine complex was then measured by flame atomic absorption spectrometry. The concentration of depleted copper was proportional to the concentration of melamine, with a linear calibration curve (melamine concentration against copper absorbance) between 0.5 and 2.5 m mol L−1 (R2 = 0.9943) and with a limit of detection of 0.50 m mol L−1. Although external standard calibration provided poor recoveries in fortified fish flesh (40% to 74% for 5 to 10 mg melamine/g), the method of standard addition provided acceptable values (90% to 93%), with a relative standard deviation of 3% to 10%. The results obtained with the standard addition method were in broad agreement with those obtained by high performance liquid chromatography. |
| Databáze: | OpenAIRE |
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