Novel polymorphic microsatellite markers for paternity analysis in the red-capped robin (Petroica goodenovii: Aves)
| Autor: | Damian K. Dowling, Gregory J. Adcock, Raoul A. Mulder |
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| Rok vydání: | 2003 |
| Předmět: |
Genetics
Ecology biology Emberiza schoeniclus biology.organism_classification Biochemistry General Biochemistry Genetics and Molecular Biology Loss of heterozygosity Petroica goodenovii Plumage Genetic marker Evolutionary biology Polymorphic Microsatellite Marker Microsatellite Phylloscopus occipitalis |
| Zdroj: | Molecular Ecology Notes. 3:517-519 |
| ISSN: | 1471-8286 1471-8278 |
| DOI: | 10.1046/j.1471-8286.2003.00497.x |
| Popis: | Seven microsatellite loci were isolated and characterized from the red-capped robin Pet-roica goodenovii , using nonradioactive polymerase chain reaction (PCR)-based techniquesto screen an enriched genomic library. Five loci showed no evidence of null alleles and werevariable [mean heterozygosity ( H E ) = 0.440, mean number of alleles = 8]. Cross-amplificationusing primers for microsatellites in Phylloscopus occipitalis and Emberiza schoeniclus yielded another two polymorphic loci. The combined set of five red-capped robin and twocross-amplified loci are suitable for paternity assignment (exclusion probability for sevenunlinked loci = 0.9760). Keywords : microsatellite, parentage, Petroica goodenovii , red-capped robin Received 15 May 2003; revision received 29 June 2003; accepted 29 June 2003 During the past decade, genetic markers have been widelyapplied to resolve parentage in populations of wild ani-mals. These studies have revealed unexpected variationin reproductive success among members of socially mono-gamous species. In birds, this variation occurs becausepaired males obtain extra-pair fertilizations (EPFs; Birkhead& Moller 1992 ), thereby enhancing their own reproductivesuccess at the expense of the cuckolded individual.Across bird species, variation in the frequency of extra-pair paternity is associated with the expression of malesecondary sexual characters. Strong sexual dichromatismin plumage (where males have more brightly colouredplumage than females) is correlated with high rates ofextra-pair paternity (Moller 1997; Owens & Hartley 1998).Therefore, objective measurement of reproductive successusing molecular tools is essential for studies that seekto examine whether a potential ornament is maintainedthrough sexual selection.The red-capped robin ( |
| Databáze: | OpenAIRE |
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