Effect of Encapsulation into Alginate Microspheres on Viability of Mesenchymal Stromal Cells after Exposure with Penetrating Cryoprotectants

Autor:	Vedeney S. Zaikov, Aleksandr Yu. Petrenko, Dmitriy N. Tarusin
Rok vydání:	2016
Předmět:	0301 basic medicine 030102 biochemistry & molecular biology Cryoprotectant Dimethyl sulfoxide Diol Mesenchymal stem cell Biophysics Medicine (miscellaneous) 03 medical and health sciences chemistry.chemical_compound chemistry Cytotoxic T cell Vitrification Cytotoxicity Ethylene glycol
Zdroj:	Problems of Cryobiology and Cryomedicine. 26:213-220
ISSN:	2518-7376 2307-6143
DOI:	10.15407/cryo26.03.213
Popis:	The effect of encapsulation into alginate microspheres (AMS) on viability of mesenchymal stromal cells (MSCs) after exposure to the solutions of penetrating cryoprotectants dimethyl sulfoxide (DMSO), ethylene glycol (EG) and 1,2-propane diol (1,2‑PD) with concentrations varying from 1.5 to 9 M during 1.5 and 5 min was studied. It was shown that the viability of MSCs both as a suspension and encapsulated in AMS decreased with a rise in cryoprotectant concentration and exposure time. Encapsulation into AMS significantly protected the cells from cytotoxic damage of the cryoprotectants, e. g . if the 5-min exposure to 9 M DMSO, EG and 1,2-PD resulted in the death of quite all the cells in the suspension, the viability of encapsulated MSCs after exposure under similar conditions made 60, 80 and 52% respectively. It was found that the studied penetrating cryoprotectants had a various cytotoxicity to MSCs, and it decreased in the row DMSO > 1,2-PD > EG. The findings can be used to develop a multi-component vitrifying solution. Probl Cryobiol Cryomed 2016; 26(3): 213–220
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::33ede6bdbb2169a718def9117cca0dfd https://doi.org/10.15407/cryo26.03.213 Zobrazit plný text záznamu