| Popis: |
Metabolomics approaches are categorized into two analytical platforms, untargeted, which offers a comprehensive scanning of the metabolome formulating the hypothesis, while targeted metabolomics explores the generated question toward validation. The identification of the metabolic entities can be intricating, due to the physicochemical diversities of the metabolites, the interlaboratory variations, and the unknown actual metabolic spectrum. Nuclear magnetic resonance and mass spectrometry detectors, coupled with liquid chromatography or gas chromatography, include the state-of-the-art methodologies of the laboratory setting for both targeted and untargeted metabolomics studies. These analytical techniques can provide detailed characterization of hundreds to thousands of metabolites that assist research efforts for metabolomics-based biomarker discovery of clinical relevance enabling the transition to precision medicine strategies. However, the integration of metabolic biomarkers to clinical applications entails challenges in terms of validation, which is discussed hereafter, along with the future perspectives on clinical metabolomics translational potentials. |
