Involvement of the Rcs regulon in the persistence ofSalmonellaTyphimurium in tomatoes
Autor: | Max Teplitski, Marcos H. de Moraes, M. A. Farias, Steffen Porwollik, Massimiliano Marvasi, Michael McClelland, Isai Salas-González |
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Rok vydání: | 2016 |
Předmět: |
0301 basic medicine
Salmonella biology fungi 030106 microbiology Mutant Protein Data Bank (RCSB PDB) Wild type food and beverages Human pathogen biology.organism_classification medicine.disease_cause Agricultural and Biological Sciences (miscellaneous) Microbiology 03 medical and health sciences 030104 developmental biology Regulon medicine Gene Ecology Evolution Behavior and Systematics Bacteria |
Zdroj: | Environmental Microbiology Reports. 8:928-935 |
ISSN: | 1758-2229 |
Popis: | It is becoming clear that human enteric pathogens, like Salmonella, can efficiently colonize vegetative and reproductive organs of plants. Even though the bacterium's ability to proliferate within plant tissues has been linked to outbreaks of salmonellosis, little is known about regulatory and physiological adaptations of Salmonella, or other human pathogens, to their persistence in plants. A screen of Salmonella deletion mutants in tomatoes identified rcsA and rcsB genes as those under positive selection. In tomato fruits, populations of Salmonella rcsB mutants were as much as 100-fold lower than those of the wild type. In the follow-up experiments, competitive fitness of rcsA and rcsB mutants was strongly reduced in tomatoes. Bioinformatics predictions identified a putative Salmonella RcsAB binding box (TTMGGAWWAABCTYA) and revealed an extensive putative RcsAB regulon, of which many members were differentially fit within tomatoes. |
Databáze: | OpenAIRE |
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